Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.208242 |
Chromosome: | chromosome_7 |
Location: | 5177227 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g348450 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCAATACATTGCTTCGGCGTGTGGGGACG |
Internal bar code: | GGTGAAGACGCGTTAGCGGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 599 |
LEAP-Seq percent confirming: | 98.3425 |
LEAP-Seq n confirming: | 1780 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCGGAGTGTCACGTTATTT |
Suggested primer 2: | GAAGCTAGGTGGCATGAAGC |