Insertion junction: LMJ.RY0402.208242_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208242
Chromosome:chromosome_7
Location:5177227
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTCAATACATTGCTTCGGCGTGTGGGGACG
Internal bar code:GGTGAAGACGCGTTAGCGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:98.3425
LEAP-Seq n confirming:1780
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGGAGTGTCACGTTATTT
Suggested primer 2:GAAGCTAGGTGGCATGAAGC