| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208245 |
| Chromosome: | chromosome 13 |
| Location: | 1577567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573150 | FPN5 | Purine 5'-nucleotidase; (1 of 1) PTHR12103:SF15 - PROTEIN Y71H10B.1, ISOFORM C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCAACCTGATCAAGGTGGACAGATT |
| Internal bar code: | CTACTGCGACGTCGTAGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 98.9904 |
| LEAP-Seq n confirming: | 34316 |
| LEAP-Seq n nonconfirming: | 350 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGCTTTGCAAGAATAAA |
| Suggested primer 2: | ATACACTCTCCTGCGCGTCT |