Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.208273 |
Chromosome: | chromosome 3 |
Location: | 2590152 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g160550 | MTP4 | Metal Transport Protein 4; (1 of 3) PTHR11562:SF21 - PROTEIN R02F11.3, ISOFORM B | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGTCGCGCCAGTGGTTCCGGACAGCT |
Internal bar code: | CCATTAGGTCTGCCACGGGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 833 |
LEAP-Seq percent confirming: | 99.2786 |
LEAP-Seq n confirming: | 8257 |
LEAP-Seq n nonconfirming: | 60 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTAAACACGGGTCCAAAGG |
Suggested primer 2: | TCATCAGATAGCTTGTGGCG |