| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208273 |
| Chromosome: | chromosome 5 |
| Location: | 347236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233303 | (1 of 1) K11718 - UDP-glucose:glycoprotein glucosyltransferase (HUGT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGCTGTTTCGTGGCAGTGGGCGGCGC |
| Internal bar code: | TTCATGTGGGGTGCTCGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 59.5463 |
| LEAP-Seq n confirming: | 7008 |
| LEAP-Seq n nonconfirming: | 4761 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTAAACCAAAGCCA |
| Suggested primer 2: | CTTGTCCTTGTTGCCCTTGT |