| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208291 |
| Chromosome: | chromosome 3 |
| Location: | 7945534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202150 | DMC2 | (1 of 6) 2.1.1.37 - DNA (cytosine-5-)-methyltransferase / Type II DNA methylase; Putative DNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTGTTCTTAAATTCATTGCGGCTTGC |
| Internal bar code: | CGGTTCAATGGGAAGGTCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1051 |
| LEAP-Seq percent confirming: | 98.3498 |
| LEAP-Seq n confirming: | 1192 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAAACACGCTCTGACGC |
| Suggested primer 2: | GAGACATTGGTCGTCAGGGT |