| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208291 |
| Chromosome: | chromosome 3 |
| Location: | 7945535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202150 | DMC2 | (1 of 6) 2.1.1.37 - DNA (cytosine-5-)-methyltransferase / Type II DNA methylase; Putative DNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCGAACGTAAAACTCTCGCGACACCA |
| Internal bar code: | GATTACCCCCTAGTTGTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACATTGGTCGTCAGGGT |
| Suggested primer 2: | TTGTAAACACGCTCTGACGC |