| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208295 |
| Chromosome: | chromosome 2 |
| Location: | 1902333 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087700 | APX1 | (1 of 1) PTHR31356//PTHR31356:SF1 - FAMILY NOT NAMED // L-ASCORBATE PEROXIDASE S, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Ascorbate peroxidase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGAGATTGACCATGGCGCCAACAAGGG |
| Internal bar code: | AACGTTGTGCTGTACATGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 98.8488 |
| LEAP-Seq n confirming: | 3778 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCGTAATCATCTGCAA |
| Suggested primer 2: | AGACCCATGCGGTAGAACAC |