| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208324 |
| Chromosome: | chromosome 7 |
| Location: | 934483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACCGCTCCTTCCATGCCCCACTCCTC |
| Internal bar code: | TGCACGGCCAACAGGAATGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 94.6092 |
| LEAP-Seq n confirming: | 351 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACGTACGGTGACTAGGT |
| Suggested primer 2: | AATGCAATTGTCTTCGGGAC |