| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208344 |
| Chromosome: | chromosome 7 |
| Location: | 5590746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351825 | (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAACAATCGGCGCCTCCGCGTCCGCTG |
| Internal bar code: | AGTTCCAGGACACGAGGTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.4191 |
| LEAP-Seq n confirming: | 3252 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCGAGCTGTAAATAGCC |
| Suggested primer 2: | CCACGCCTTACCTATGCAAT |