| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208350 |
| Chromosome: | chromosome 6 |
| Location: | 3844967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278172 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACGGCGCTCTCACGCCGCGCACACACA |
| Internal bar code: | TTATGTTATCTGGGTAATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 22.1352 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 795 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCCTGCTCTCATTCAG |
| Suggested primer 2: | AGTTGCCTCGCCATTATTTG |