Insertion junction: LMJ.RY0402.208358_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.208358
Chromosome:chromosome 4
Location:1739350
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g212350 (1 of 2) PF10013 - Uncharacterized protein conserved in bacteria (DUF2256) (DUF2256) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTGGCGCACACCTGGGGACGAGGGGGG
Internal bar code:CCGGACGATTTTGCAGTATGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:99.7527
LEAP-Seq n confirming:1210
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGAAAGCAAAAAGGGTA
Suggested primer 2:TCATGCTTGGCAGTAAGCAC