Insertion junction: LMJ.RY0402.208370_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208370
Chromosome:chromosome 7
Location:2929927
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGTACGCGGCGGCGATGCGGCAGCAGCA
Internal bar code:CGTTACGCCACAAGGCGGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:668
LEAP-Seq percent confirming:99.871
LEAP-Seq n confirming:2322
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AATTTGGTCTTATGCACGGC
Suggested primer 2:TTACATTGCCGCTGTAGTGC