Insertion junction: LMJ.RY0402.208389_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208389
Chromosome:chromosome 11
Location:2623072
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476376 FAP221,Pcdp1,RPC82 Flagellar Associated Protein 221; (1 of 1) PTHR23053//PTHR23053:SF21 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTCCACGTCCAGGCTGCCGTACGGGCTG
Internal bar code:TTGAGGCGCAGGATACCATGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:99.795
LEAP-Seq n confirming:13141
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCGGTAAAGGAGGGGTTT
Suggested primer 2:ATACCAGCCCAAACTGAACG