Insertion junction: LMJ.RY0402.208396_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.208396
Chromosome:chromosome 1
Location:3633378
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g023400 (1 of 1) K14617 - LMBR1 domain-containing protein 1 (LMBRD1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGCATTCGCTAACTCTGTCTGACTCTT
Internal bar code:TTTTGGCATCTTTGTAACTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:98.8417
LEAP-Seq n confirming:256
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCCCTGTGCTTTCTAACT
Suggested primer 2:CGTGGGTGACATACTCATCG