| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208401 |
| Chromosome: | chromosome_1 |
| Location: | 5145891 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g035800 | IRK2 | Inwardly-rectifying potassium channel | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCCCGCAGCGGTGCGGGTTCAAGGGGGA |
| Internal bar code: | AAATCCGCATAACACACGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGGGGTAAGACTTTTC |
| Suggested primer 2: | GTCGCGTGGTAAATGTCCTT |