| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208401 |
| Chromosome: | chromosome_1 |
| Location: | 5145912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g035800 | IRK2 | Inwardly-rectifying potassium channel | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGAACATGACAATGGGTGTGAGACCGCGC |
| Internal bar code: | TATCTGTGCTCATCATGATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.6654 |
| LEAP-Seq n confirming: | 5064 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCGTGGTAAATGTCCTT |
| Suggested primer 2: | GGGCAGGGGTAAGACTTTTC |