Insertion junction: LMJ.RY0402.208401_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208401
Chromosome:chromosome 13
Location:3742241
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGGTTGCCACGTCGCCTGCTGAGCCCA
Internal bar code:GGGTAACCCCCGGGATCGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:841
LEAP-Seq percent confirming:99.961
LEAP-Seq n confirming:2563
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:CACAACTCGCTTTCACTCCA