| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208402 |
| Chromosome: | chromosome 12 |
| Location: | 6889490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560800 | (1 of 2) K14664 - IAA-amino acid hydrolase [EC:3.5.1.-] (ILR1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTCGAGCACCGACATGAAGTTAATCG |
| Internal bar code: | ACGCACACGCGGGGGGGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTCCCGTTCCAGGAAGT |
| Suggested primer 2: | GCAGCCTGTGAGACAGTGAG |