Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.208420 |
Chromosome: | chromosome 6 |
Location: | 3519620 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278108 | (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) | intron|outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTAAGTATGCTTAGGATGGCGCCGGCG |
Internal bar code: | GTGTTAGTTTGGCCCCGCAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 834 |
LEAP-Seq percent confirming: | 99.7358 |
LEAP-Seq n confirming: | 2643 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTTCACCTGTTGGGTCGTC |
Suggested primer 2: | GAAGACACGGGTAGCAGGAG |