| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208431 |
| Chromosome: | chromosome 5 |
| Location: | 3358909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240750 | TEF17 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGGGATGGCAAGGCGATGACGTTGTT |
| Internal bar code: | GCACACCAAAGGACGCGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.7775 |
| LEAP-Seq n confirming: | 2690 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTCAACCGTGGGAAGAC |
| Suggested primer 2: | ATTTTGCCGACCTGAGTGAC |