| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208431 |
| Chromosome: | chromosome_7 |
| Location: | 5972603 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g354551 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGCGGGGACTCGAGCGGAGACGGCTGAG |
| Internal bar code: | GAGCAAGTCCTCGGCGACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 103 |
| LEAP-Seq percent confirming: | 97.5309 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGAGAGAAAGGAAACG |
| Suggested primer 2: | GATGAGACCGGTGAGCGTAT |