Insertion junction: LMJ.RY0402.208440_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.208440
Chromosome:chromosome 10
Location:6022006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g463026 (1 of 1) IPR001202//IPR001752//IPR027417//IPR027640 - WW domain // Kinesin motor domain // P-loop containing nucleoside triphosphate hydrolase // Kinesin-like protein; Large protein with small region (p-loop?) Similar to kinesin (peptide is unique) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTCGCACACACGCGCCTGCTCGCTCC
Internal bar code:CGTCTGCGGATCTTATTCGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:989
LEAP-Seq percent confirming:89.3337
LEAP-Seq n confirming:3191
LEAP-Seq n nonconfirming:381
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTAGTTTGGGCTGGTA
Suggested primer 2:TGCGAGTGAGTGTGTGATGA