| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208453 |
| Chromosome: | chromosome 6 |
| Location: | 2992430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTGTCAGCCCCGCCCGAATCACTTCT |
| Internal bar code: | ACGTATGATCCTCAGCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.5536 |
| LEAP-Seq n confirming: | 446 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCAAGCCCAAGGTAAG |
| Suggested primer 2: | GTGCTGTACAATGTGGGACG |