Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.208458 |
Chromosome: | chromosome_2 |
Location: | 1087257 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g081050 | FAP24 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTGGTACACGCGGCTAGGCACGGGGCGTT |
Internal bar code: | TCCTGCCTGACGCTTAGCCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 59 |
LEAP-Seq percent confirming: | 59.5745 |
LEAP-Seq n confirming: | 84 |
LEAP-Seq n nonconfirming: | 57 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCACCTACCTGTCCAAGAA |
Suggested primer 2: | CAACCTAGCCTCACAGCACA |