| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208458 |
| Chromosome: | chromosome_16 |
| Location: | 6515325 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g672800 | GMP1 | GDP-D-mannose pyrophosphorylase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCATACGCCCATCCAAGTAATCCAGTACG |
| Internal bar code: | GTATAGATATTGGTTCCATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 99.718 |
| LEAP-Seq n confirming: | 2475 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATAGCACCCAACCACCTG |
| Suggested primer 2: | CCCAACTCACCCTTCAGGTA |