| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208495 |
| Chromosome: | chromosome 11 |
| Location: | 3527700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481400 | (1 of 1) K08864 - tousled-like kinase [EC:2.7.11.1] (TLK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCCAGGCCCACCTACTCTACTCGCTTC |
| Internal bar code: | CATTAATGCTACTCAATTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGGATTTGGCGTGTTC |
| Suggested primer 2: | GTGTGGAGGTGTGGGAGAGT |