| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208499 |
| Chromosome: | chromosome 17 |
| Location: | 7029875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746647 | PUF2 | (1 of 1) PTHR12537:SF12 - MATERNAL PROTEIN PUMILIO; Puf protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGCTCCTGGCAGCTCATCTCGTCTCG |
| Internal bar code: | TCGCTCGGCAGGAGATGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.8506 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAACCCCCATGTATTTCA |
| Suggested primer 2: | GCAGTAATGGCCTAGCAAGC |