| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208515 |
| Chromosome: | chromosome 11 |
| Location: | 1285621 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467708 | (1 of 1) IPR029022 - ABC transporter, BtuC-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGGGAATGGTGGCGGAGGGGGTAGG |
| Internal bar code: | TCGGCGGCGTCATCGACTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGAAAGCACCCGAGACT |
| Suggested primer 2: | GTGTACAGCCCCACTCCACT |