Insertion junction: LMJ.RY0402.208517_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208517
Chromosome:chromosome 13
Location:4363525
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g602800 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTACCACTGTCCCATCACACACCTATCC
Internal bar code:CTCTGCGGGTCGGCCCAAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:379
LEAP-Seq percent confirming:83.3656
LEAP-Seq n confirming:862
LEAP-Seq n nonconfirming:172
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCATTGCTCCGTTCCTACAT
Suggested primer 2:GCAATAGTCGATAGGCGAGG