| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208532 |
| Chromosome: | chromosome 14 |
| Location: | 1174637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615800 | (1 of 3) PTHR31563:SF1 - ION CHANNEL POLLUX-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCAATGCGCACCTGAGCGCCGAGGA |
| Internal bar code: | TCTCAAGTGATCCAGCCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 99.2976 |
| LEAP-Seq n confirming: | 1131 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTGTTGTTGTTGGT |
| Suggested primer 2: | TTCACGGACATCGGTAACAA |