| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208543 |
| Chromosome: | chromosome 12 |
| Location: | 4748942 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523950 | (1 of 1) PTHR21229//PTHR21229:SF2 - LUNG SEVEN TRANSMEMBRANE RECEPTOR // PROTEIN C15H9.5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAATCCATGCGCCGAGTCCAATCATACG |
| Internal bar code: | ATCTCGCAAAAACACGCCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.8415 |
| LEAP-Seq n confirming: | 630 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTACCTCGCCCTTTACC |
| Suggested primer 2: | TTGGTTTGGTGGCAGTGTTA |