| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208559 |
| Chromosome: | chromosome 9 |
| Location: | 3308205 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388319 | (1 of 1) PTHR13390:SF0 - UPF0554 PROTEIN C2ORF43 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAGCGTCTTATAGCTGCCCACAAGCC |
| Internal bar code: | GCGACCGAGTTCGGCGTAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACTGGCTATGGACCGTG |
| Suggested primer 2: | AAGGTGAGAGCAGTAGGGCA |