| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208604 |
| Chromosome: | chromosome 10 |
| Location: | 3398709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAGCATTGGGCGGTTGTTTAAGGGGTT |
| Internal bar code: | GAGGCAACCATTGGGCGAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 97.677 |
| LEAP-Seq n confirming: | 883 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATTTTCATGTGCGTGG |
| Suggested primer 2: | TCGATAGACTGGTCGTGCTG |