| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208613 |
| Chromosome: | chromosome 8 |
| Location: | 620943 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359850 | FBB11 | Flagellar and Basal Body Protein 11; (1 of 2) PF13864 - Calmodulin-binding (Enkurin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGCAGGTGAAAGTTTACAAGAGTACCT |
| Internal bar code: | AATGTGTTTGAGAGTCTAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.8811 |
| LEAP-Seq n confirming: | 840 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCGAATGTGCTTACAA |
| Suggested primer 2: | GAGCTTCTTGCCAAGTGGAC |