| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208618 |
| Chromosome: | chromosome_13 |
| Location: | 5099017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g607300 | MAPK5 | Mitogen-Activated Protein Kinase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCCGGGTCGTTATCACCCAGCCACCTTG |
| Internal bar code: | AATCGGCAGAGGGCTTTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 68.0556 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTCAGTTGAGGTGGTTC |
| Suggested primer 2: | ACCAACACGCACTACAGCAG |