Insertion junction: LMJ.RY0402.208634_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.208634
Chromosome:chromosome 8
Location:741511
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCAGGCCATGATTGAGGTGCCCAAGCTG
Internal bar code:CCATACAAGGGCACGCACGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:99.8001
LEAP-Seq n confirming:2995
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGCTCCTCTGTTTCCAAC
Suggested primer 2:CGACCATCCCTGTTCTGTTT