| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208637 |
| Chromosome: | chromosome 17 |
| Location: | 7009971 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746547 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACTGATAGACTACAGCAGGATTGCAAC |
| Internal bar code: | ACCTGCTTGATTCGACTATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2727 |
| LEAP-Seq percent confirming: | 99.5033 |
| LEAP-Seq n confirming: | 10217 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCTGGTAGTTTGTTGAT |
| Suggested primer 2: | GTGGGCGACTTCTTGTTGAT |