| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208693 |
| Chromosome: | chromosome 16 |
| Location: | 2020039 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656900 | (1 of 77) IPR001623 - DnaJ domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTATAGGCGTGAGCCATATAGCCTCAT |
| Internal bar code: | GGTACACACGACCCGGAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 98.2039 |
| LEAP-Seq n confirming: | 3390 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGGAGCACAGGTAGATG |
| Suggested primer 2: | GAGAGAATTGCTGGCTGAGG |