| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208754 |
| Chromosome: | chromosome 8 |
| Location: | 828684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360850 | (1 of 1) K18798 - protein AFG1 (AFG1, LACE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGACAGGCTGTGTACATGCCAGTGGG |
| Internal bar code: | TGGCGCCCTGCGAGCTTCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 98.5386 |
| LEAP-Seq n confirming: | 472 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGGAAACGGCCAAAATG |
| Suggested primer 2: | CTCTCCTGCCCTAGACATGC |