| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208776 |
| Chromosome: | chromosome 14 |
| Location: | 3032657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628237 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGACACTTGCAACATGGGCCTCGCTAC |
| Internal bar code: | CCGTATGGGCCCGTCGGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCAGAGAAATAGCGTGG |
| Suggested primer 2: | AACCAAGCAATGCAGCTTCT |