| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208780 |
| Chromosome: | chromosome 2 |
| Location: | 984291 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080200 | TRK1 | Transketolase, chloroplastic; (1 of 1) 2.2.1.1 - Transketolase / Glycoaldehyde transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGACACTCCCATTCGAGCCAGGAGGA |
| Internal bar code: | GGGCCTGTACCCCGACTCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1339 |
| LEAP-Seq percent confirming: | 99.8696 |
| LEAP-Seq n confirming: | 2298 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGAGCATTTGTTGGTT |
| Suggested primer 2: | AAACGCTGTCTGGGGTAATG |