| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208780 |
| Chromosome: | chromosome 2 |
| Location: | 984295 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080200 | TRK1 | Transketolase, chloroplastic; (1 of 1) 2.2.1.1 - Transketolase / Glycoaldehyde transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATAAATGCCTCGGATTGAACGCCTGTAA |
| Internal bar code: | AGGTGCTATGTGTAGCGATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 77.571 |
| LEAP-Seq n confirming: | 2459 |
| LEAP-Seq n nonconfirming: | 711 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGAGCATTTGTTGGTT |
| Suggested primer 2: | AGCAACCAACGTAATGGGAG |