| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208781 |
| Chromosome: | chromosome 8 |
| Location: | 3637862 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376350 | ASF1 | Anti-silencing factor; (1 of 1) K10753 - histone chaperone ASF1 (ASF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACACGGCGCAAATTTCATGCAAACAGT |
| Internal bar code: | CCCTTCAAAGTTGAATCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 44 |
| LEAP-Seq percent confirming: | 99.8053 |
| LEAP-Seq n confirming: | 3075 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACAAGCACAGGGCTGCT |
| Suggested primer 2: | CTCCTCACCTGCTCTTACCG |