| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208789 |
| Chromosome: | chromosome 3 |
| Location: | 7502667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205400 | (1 of 1) PF01344//PF07707 - Kelch motif (Kelch_1) // BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACACGGATCCTGCCTCTCAATCTCAAA |
| Internal bar code: | GACAGCGAACAACTTCTTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 96.5271 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCTAGGGACTGTTGCC |
| Suggested primer 2: | CCCCATATACACACACAGCG |