| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208809 |
| Chromosome: | chromosome 6 |
| Location: | 1452349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATATGAGCCTGCCCGTGTGAGCTAAATTG |
| Internal bar code: | TATTGAGCCACGGGAAGGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 97.6128 |
| LEAP-Seq n confirming: | 7851 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACTGCGATGCTTCTTGT |
| Suggested primer 2: | CGTCATCGCTTAGGAAGGAG |