| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208828 |
| Chromosome: | chromosome_8 |
| Location: | 1522787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g364850 | AC115 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTCCGATCTGCCCCGGCAACATCCCCGTA |
| Internal bar code: | CCCGGTCCGACGTCATGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 85.1064 |
| LEAP-Seq n confirming: | 200 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTGTTCTGTTGTGGTCG |
| Suggested primer 2: | CGCTTTCCTGTTACTGCCTC |