| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208861 |
| Chromosome: | chromosome 12 |
| Location: | 2920487 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501800 | (1 of 1) PTHR13193:SF0 - PROTEIN ASTERIX | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTTGGCACGGGTCGCGGGGCAGGGAT |
| Internal bar code: | GCCAGCCCAGGTCCACCAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 82.5292 |
| LEAP-Seq n confirming: | 1129 |
| LEAP-Seq n nonconfirming: | 239 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGGAAAAACCCGAGAC |
| Suggested primer 2: | ACAACCTGACACACTGCTCG |