| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208868 |
| Chromosome: | chromosome 5 |
| Location: | 1082334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246100 | (1 of 1) IPR019887 - Transcription regulator AsnC-type, C-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCAAACCTCCCAAACCCCTCCCCCTCT |
| Internal bar code: | GCTGGCACTCTTATGAGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGTCCATTTCGTAACTC |
| Suggested primer 2: | AACTTCTGTGGATTGTGGGC |