| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208887 |
| Chromosome: | chromosome 7 |
| Location: | 2880975 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332300 | GWD2,1 | Alpha-glucan water dikinase 2; (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGGCAGGGCGCTCGGATGAGAGAGAC |
| Internal bar code: | ACGCGAGTGCGGCCGTGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.4675 |
| LEAP-Seq n confirming: | 1681 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGCCGTACTTCGCTAC |
| Suggested primer 2: | TGGATGCGGATTACAAGTGA |