| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208892 |
| Chromosome: | chromosome 4 |
| Location: | 281285 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217750 | (1 of 1) PTHR16017//PTHR16017:SF0 - GASTRULATION DEFECTIVE PROTEIN 1-RELATED // WD REPEAT-CONTAINING PROTEIN 70 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACATGAGATGGGGGGCGGGGAAGTCGG |
| Internal bar code: | GAGCCCCTAAGCTGGGGGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 99.7795 |
| LEAP-Seq n confirming: | 905 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGGAGCAGTCCAGAG |
| Suggested primer 2: | TCTCCAAATTCAGAATCCCG |